DPPH RADICAL-SCAVENGING ACTIVITY OF NOODLES, STICKS, AND COOKIES INCORPORATED WITH CLITORIA TERNATEA EXTRACT
DOI:
https://doi.org/10.60050/lkh.v10i2.126Keywords:
Antioxidant Activity, Butterfly Pea Flower, Clitoria Ternatea, DPPH, Functional FoodAbstract
Background: Butterfly pea flowers (Clitoria ternatea L.) contain bioactive compounds - specifically anthocyanins - that possess antioxidant properties and have potential applications as functional food ingredients. However, different food processing methods can influence the antioxidant activity present in the final product.
Objectives: This study aimed to compare the antioxidant activity of butterfly pea flower extract incorporated into three food products, namely, noodles, sticks, and cookies.
Methods: This study employed a laboratory experimental design to evaluate the antioxidant activity of butterfly pea floer- based food products. Samples of butterfly pea flower noodles, sticks, and cookies wew dried, ground into powder, and extracted using methanol. The filtrates were reacted with 2,2-diphenyl-1-picrylhydrazy (DPPH) solution and incubated for 30 minutes in the dark at room temperature. Antioxidant activity was determined by measuring absorbance at 517 nm using a UV-Visible spectrophotometer and expressed as the percentage of DPPH radical scavenging activity.
Results: Butterfly pea flower sticks exhibited the highest DPPH radical-scavenging activity at 10.46%, followed by cookies at 8.66% and dried noodles at 3.94%. The results indicate differences in DPPH radical-scavenging activity among the three food products, which may be associated with differences in formulation, food matrix, processing conditions, and extraction efficiency.
Conclusion: Among the tested products, butterfly pea flower sticks demonstrated the highest DPPH radical-scavenging activity. The findings indicate that product type and processing conditions may influence the retention and measurable activity of bioactive compounds from butterfly pea flower. However, the observed antioxidant activities were relatively low and should be interpreted within the specific testing conditions. Further research using multiple sample concentrations is recommended to determine the concentration-response relationship and IC50 value.
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